METHOD FOR IN VITRO MULTIPLICATION OF GLADIOLUS Russian patent published in 2006 - IPC A01H4/00 A01H5/00 

FIELD: cultivation of orchards, in particular, in vitro micromultiplication and sanitation of gladiolus plants.

SUBSTANCE: method involves separating explants; planting onto Murasige-Scuga nutrient medium; cultivating and dividing into segments; after separation, planting explants onto agarized Murasige-Scuga nutrient medium with 1/4 concentration of macro- and micro salts at constant illumination by means of luminescent lamps having intensity of 3,000-3,500 lux at temperature of 22-24 C; transferring multiplied explants onto agarized nutrient medium with 1/2 concentration of salts by Murasige-Scuga prescription; supplementing with 30-40 g/l of saccharose and 0.5-1.0 mg/l of growth promoters of 6-benzylaminopurine and 0.5-1.0 mg/l of alpha-naphthaleneacetic acid; simultaneously providing cultivation through callusogenesis on agarized nutritive medium supplemented with 0.5-1.0 mg/l of 6-benzylaminopurine and 1.0-1.5 mg/l of 2,4-dichlorophenoxyacetic acid, with following rooting on agarized nutritive medium at half the concentration of salts by Murasige-Scuga prescription, supplemented with 0.5-1.5 mg/l of alpha-naphthalenecetic acid, 30-40 g/l of saccharose and 4-5 g/l of activated carbon.

EFFECT: increased yield of multiplied material due to increased budding intensity, improved growth and development of explants.

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