METHOD FOR GROWING PLANT RAW MATERIALS OF SIBERIAN IRIS (IRIS SIBIRICA L.) WITH A GIVEN CONTENT OF EXTRACTIVES Russian patent published in 2022 - IPC A01H4/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology and can be used for the industrial production of high-value raw materials in order to obtain medicines, biologically active additives, functional foods, therapeutic and preventive cosmetics and individual biologically active substances. The invention is a method for obtaining plant raw materials of Siberian iris (Iris sibirica L.) by clonal micropropagation and cultivation under hydroponic conditions with a given content of extractive substances, including the introduction of explants into culture in vitro, cultivation on MS nutrient medium to obtain regenerants, their reproduction and rooting, according to the invention, cultivation is carried out on a three-tiered universal aeroponic installation filled with a nutrient solution according to the Murashige-Skoog recipe with a concentration of macro-, micro-salts, calcium chloride and iron chelate with the addition of 10.0 mcM of benzylaminopurine + 1.0 mcM of naphthyl acetic acid + 0.1 mcM of isopropyl formic acid to obtain raw materials with extractive substances content of 15.5 ± 0.5% on absolutely dry raw materials (on a.d.r.m.) and flavonoids 6.9 ± 0.9% on a.d.r.m., or 5.0 mcM of benzylaminopurine to obtain raw materials with a content of hydroxycinnamic acids of 5.19 ± 0.09% per a.d.r.m. and tannins of 2.0 ± 0.3% per a.d.r.m.

EFFECT: output of plant raw materials of Siberian iris with a given content of flavonoids, hydroxycinnamic acids and tannins is carried out.

1 cl, 1 dwg, 1 tbl, 4 ex