FIELD: method for culturing of ovarian follicles in vitro for biological investigation.
SUBSTANCE: claimed method includes the next subsequent steps: vessel providing for culturing in vitro; follicle isolation from mammalian ovary containing at least theca cells or pre-theca cells, granulosis cells and oocyte; optionally the first culturing stage using oil-free first medium to inhibit an attachment; the second culturing stage using oil-free second medium to provide attachment; and producing of mature follicle.
EFFECT: method for production of oocytes with high fertilization effectiveness and for evaluation of environmental factor influence on oocytes.
5 tbl, 5 dwg, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| TECHNOLOGY FOR HUMAN OOCYTE MATURATION AT GV STAGE USING EXTRACELLULAR FOLLICULAR FLUID VESICLES IN FERTILIZATION PROGRAMS IN VITRO: V-IVM (EXTRACELLULAR VESICLES IN VITRO MATURATION) | 2023 |
|
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| METHOD FOR EXTRACORPOREAL CULTIVATING BOVINE OOCYTES | 2009 |
|
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| METHOD OF PRODUCING CATTLE EMBRYOS IN VITRO | 2023 |
|
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| DIAGNOSTIC TECHNIQUE FOR REPRODUCTIVE OVARIAN POTENCY | 2008 |
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| AGENT FOR TREATING FEMALE INFERTILITY AND INFERTILITY OF FEMALE ANIMALS | 2017 |
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RU2740922C2 |
| NUTRIENT MEDIUM FOR THE CULTIVATION OF BOVINE OOCYTES IN VITRO | 2021 |
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RU2778147C1 |
