FIELD: medicine.
SUBSTANCE: invention refers to medicine and biology, namely to sterility diagnostics. There is offered method for evaluating the reproductive ovarian potency. Ovarian tissue is fixed in Bouin's fixative solution; histological sections of thickness 7-8 mcm are prepared and stained in hematoxylin and eosin. A portion of germ cells is evaluated for each developmental stage from total gamete count: for oocytes in %% of total oocyte count; for follicles and derivatives - count at each stage per 1 microscopic field. The control indices of various oogenesis and folliculogenesis stages are determined in healthy representatives of different mammals. The reproductive ovarian potency and potential pathology are estimated after considering the results of comparison to the control indices.
EFFECT: possibility to diagnose impaired oogenesis and folliculogenesis infringements in gynaecology, veterinary science and livestock sector.
4 dwg, 8 tbl
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Authors
Dates
2009-09-20—Published
2008-04-03—Filed