FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. The invention is a method, which consists in the fact that the egg is placed in a nutrient medium for in vitro maturation, where it matures to an oocyte ready for fertilization within 24 hours in a medium in which the volume concentration of CO2 is 6%. Culture medium for in vitro egg maturation consists of TCM-199, 10% FBS, 5mg/ml FSH-super, 50mg/ml gentamicin, 3mg/ml B12, 4mM L-carnitine.
EFFECT: improving the quality and viability of bovine oocytes.
1 cl, 2 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PRODUCING IN VITRO CATTLE EMBRYOS | 2023 |
|
RU2823596C1 |
| METHOD OF PRODUCTION OF SHEEP EMBRYOS in vitro | 2013 |
|
RU2525714C1 |
| METHOD FOR EXTRACORPOREAL CULTIVATING BOVINE OOCYTES | 2009 |
|
RU2410063C1 |
| METHOD OF PRODUCING CATTLE EMBRYOS IN VITRO | 2023 |
|
RU2818068C1 |
| METHOD FOR PIG EMBRYONS GENERATION IN VITRO | 2007 |
|
RU2340177C1 |
| METHOD OF OBTAINING OF HYBRID HUMAN STEM CELL | 2007 |
|
RU2352637C1 |
| METHOD FOR EXTRACORPOREAL CULTIVATION OF OOCYTES IN COWS | 2015 |
|
RU2602448C1 |
| TECHNOLOGY FOR HUMAN OOCYTE MATURATION AT GV STAGE USING EXTRACELLULAR FOLLICULAR FLUID VESICLES IN FERTILIZATION PROGRAMS IN VITRO: V-IVM (EXTRACELLULAR VESICLES IN VITRO MATURATION) | 2023 |
|
RU2807492C1 |
| METHOD OF USING EXTRACELLULAR FOLICULAR FLUID VESICLES TO INCREASE HUMAN SPERMATOZOA MOBILITY IN VIRTUE FERTILIZATION PROGRAMS | 2022 |
|
RU2801339C1 |
| METHOD FOR ISOLATION AND MATURATION OF IMMATURE OOCYTE-CUMULUS COMPLEXES FROM OVARIAN TISSUE AFTER OOPHORECTOMY | 2020 |
|
RU2745425C1 |
