METHOD FOR OBTAINING PREPARATION THAT CONTAINS THE COMPLEX OF HEAT SHOCK PROTEINS 70, 90, 96 ASSOCIATED WITH ANTIGENOUS PEPTIDES Russian patent published in 2006 - IPC A61K38/02 A61K35/12 A61P35/00 

FIELD: biopharmacology, preparative biochemistry and medicine.

SUBSTANCE: the method deals with homogenizing mammalian tissues and centrifuging. It is necessary to add ammonium sulfate to supernatant that contains the complex of heat shock proteins (HSP) up to 35-65% saturation, the residue developed should be dissolved in phosphate buffer at pH 7.2 followed by chromatography upon a column with sephadex G-50, then obtained protein fraction should be exposed to the action of chromatography upon heparin-sepharose. Fractions that contain TSP 70, 90, 96 the content of which was detected with the help of corresponding antibodies should be united, concentrated and salted out due to ultrafiltration. The innovation provides more simplified and shortened terms for implementing the method without applying expensive equipment. The complexes obtained should be applied in immunotherapy of oncological diseases.

EFFECT: higher efficiency.

2 dwg, 1 tbl