FIELD: medicine.
SUBSTANCE: invention can be used to isolate tumour antigens for tumour immunotherapy. For this, 1 gram of tumour is chopped on a cooled surface, 9 ml of buffer (pH 7.0) consisting of 10 mM of NaHCO3, 10 mM of EDTA, 2 mM of PMSF is added, and homogenized. Then it is centrifuged first at 20,000 g at 4°C for 30 minutes, and then at 100,000 g at 4°C for 90 minutes. The supernatant is subjected to ultra-/diafiltration using a membrane with a cut-off limit of 50 kDa, using a formulation buffer (pH 7.0) consisting of 10 mM of NaHCO3 and 150 mM of sodium chloride.
EFFECT: obtaining of a fraction consisting of complexes represented by different classes of heat shock proteins, which determines a wide range of associated tumour peptides.
1 ex, 2 tbl, 2 dwg
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