METHOD OF DETERMINING 2,4,6-TRINITROMETHYLBENZENE IN BIOLOGICAL MATERIAL Russian patent published in 2008 - IPC G01N30/02 B01J20/283 G01N31/00 

FIELD: analytical methods in toxicology and sanitary chemistry.

SUBSTANCE: sample of biological material is milled and infused two times with acetonitrile (each time for 45 min). Extracts are separated from solid biological material particles, combined, and vaporized in air flow at 16-22°C. Dry residue is dissolved in hexane-acetone (8,5:1.5) solvent mixture and subjected to chromatographic analysis in column with silica gel L 40/100 μm using hexane-acetone (8,5:1.5) as mobile phase. Eluate fractions containing 2,4,6-trinitromethylbenzene are combined, eluent is evaporated, and residue dissolved in solvent system acetonitrile-water (4:6). Resulting solution is subjected to HPLG (high-performance liquid chromatography) analysis in column filled with sorbent Nova Pack C-18 using acetonitrile-water (4:6) as mobile phase and diode-matrix detector at column temperature 20°C and mobile phase supply velocity 1 mL/min.

EFFECT: increased accuracy and sensitivity of analysis and accelerated analytical procedure.

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