METHOD FOR MANUFACTURING ASSOCIATED INACTIVATED CULTURE VACCINE AGAINST INFECTIOUS RHINOTRACHEITIS AND PARAGRIPPE-3 IN CATTLE Russian patent published in 2006 - IPC A61K39/295 A61K39/265 A61K39/155 C12N7/00 

FIELD: biotechnology, veterinary virology.

SUBSTANCE: the present innovation deals with elaborating epizootically and ecologically safe associated inactivated vaccine that creates tense immunity simultaneously against IRT and PG-3 in cattle. The method deals with cultivating vaccine strains of IRT and PG-3 viruses in a cell culture, sampling virus-containing culture liquid, inactivation of virus and addition of an adjuvant. Moreover, as vaccine strains it is necessary to apply the strain of TC-A/C of IRT virus grown in passage cell culture MDVC, at activity of 7.5-8.0 lg TCD₅₀/cu. cm, and the strain PG/C-2002 of PG-3 virus grown in passage cell culture PC at activity of 7.5-8.0 lg TCD₅₀/cu. cm. On sampling virus-containing culture liquid one should carry out inactivation of virus of every strain with theotropin for about 5-7 d at 36.5-37.5° C, pH being 7.2-7.5. Moreover, one should add theotropin to virus-containing liquid of every strain at final concentration of 0.1%, and then mix inactivated virus-containing liquids of both strains at the ratio of 1:1 and add adjuvant at the quantity of 20% that consists of 6%-aluminum hydroxide and saponin gel. The innovation enables to obtain epizootically and ecologically safe vaccine that creates tense immunity in vaccinated animals, being stable at storage.

EFFECT: higher efficiency.

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