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2 289 627

(13)

(51)

IPC

C12Q1/68(2006-01-01)

C12N15/34(2006-01-01)

(21) (22)

Application

2004105688/13, 2004-02-25

(24)

Start date

2004-02-25

(22)

Actual filing date

2004-02-25

(45)

Published

2006-12-20

(72)

Inventor

Ivanchuk Igor' Ivanovich

(73)

Holder

Gosudarstvennoe Obrazovatel'Noe Uchrezhdenie Vysshego Professional'Nogo Obrazovanija Sibirskij Gosudarstvennyj Meditsinskij Universitet Gou Vpo Sibgmu

METHOD AND KIT FOR DETECTION OF LYME BORRELIOSIS BACTERIA (Borrelia burgdorferi) DNA Russian patent published in 2006 - IPC C12Q1/68 C12N15/34

Abstract RU 2289627 C2

FIELD: medicine diagnosis, molecular biology.

SUBSTANCE: invention relates to method for detection of DNA of human Lyme borreliosis excitant in peripheral blood, cell culture and mites. Claimed method includes DNA isolation from biological material; synthesis of primers; PCR with using synthesized primers and evaluation of obtained data. In method BBs14.F/BBs14.R - 5'-AAGAATACATTAAGTGCGATANN-3', 5'-CAATCCACTTAATTTTGTGTTAT-3' primers to gene site encoding 16S rRNA are used. Kit for this method also in disclosed.

EFFECT: high-specific, sensitive and simplified method.

2 cl, 2 dwg, 5 tbl

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RU 2 289 627 C2

Authors

Ivanchuk Igor' Ivanovich

Dates

2006-12-20—Published

2004-02-25—Filed