SET OF REAGENTS FOR THE DETECTION OF PATHOGENS OF TICK-BORNE BORRELIOSIS Russian patent published in 2021 - IPC C12Q1/68 

FIELD: medical biotechnology.

SUBSTANCE: invention relates to the field of medical biotechnology. Invention is a set of reagents for detecting pathogens of tick-borne borreliosis, containing DNA polymerase, buffer for isothermal reaction, the following external and internal primers for detecting Borrelia burgdorferi s DNA. l.:

F3: 5’-GCTGTTGAGCTCCTTCTTG-3’;

B3: 5’-CACCAGCGTCACTTTCAG-3’;

FIP: 5’-TGCAAATCTATTCTCTGGCGAAGGTTGAGCACCTTCTTGAACA-3’;

BIP: 5’-ACAGCAATCGCTTCATCTTGATTCTCAAGCTTCTTGGACC-3’, - new is that it additionally includes the following loop primers for detecting Borrelia burgdorferi s DNA. l.:

LF: 5'-TGCTCCCACATGAACTCTTAA-3 ';

LB: 5'-AAGGAGCTCAGGCTGCTCAGA-3 ',

primers for detecting Borrelia miyamotoi DNA:

F3: 5’-TTCTAACAAAGGACAATATTCCT-3’;

B3: 5’-TTCATTTAAGGGGAAACGATT-3’;

FIP: 5’-CGTTTTCTCTAGCTCGATTGGGAAACACGACCCAGAAATTGACA-3’;

BIP: 5’-CGATATTACGCCACTGACTTCACATGGTTTTTTGTTTTCAGGATCA-3’;

LF: 5’-TGTGCAACATTTGTGGTTG-3’;

LB: 5’- TCACTAAGTCTCAGTGAAAGA-3’,

DNA polymerase Bst 2.0 or Bsm and the corresponding isothermal Bst 2.0 or Bsm buffer, as well as MgSO₄ or MgCl₂, respectively, a mixture of dNTP nucleotides, betaine, deionized water, positive and negative controls, as well as fluorescent dyes EVA green or ethidium bromide.

EFFECT: invention makes it possible to increase the rapidity and specificity of the analysis for the detection of tick-borne borreliosis, to increase the reliability of the diagnosis by expanding the list of simultaneously determined infectious agents, as well as the possibility of performing the analysis in the field, incl. on whole ticks.

1 cl, 3 dwg, 3 ex