FIELD: medicine, immunology, cytokines.
SUBSTANCE: method for preparing interleukin-8 (L-8) from donor blood neutrophiles involves isolating neutrophiles from peripheral blood in double gradient ficol-verografin, bringing about the neutrophile content in cell suspension to the concentration 5 x 106 cells/ml, activation at temperature 37°C for 1 h followed by centrifugation and separation of supernatant containing the end product. For activation the cell suspension of neutrophiles in the concentration 5 x 106/ml of Henx's solution is poured in plastic Petri dishes. Invention provides the development of a simple and available method providing the high level of interleukin-8 (IL-8) from neutrophiles of donor blood not requiring using expensive preparations.
EFFECT: improved preparing method.
1 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF OBTAINING TUMOR NECROSIS FACTOR-Α FROM BLOOD NEUTROPHILS OF DONORS | 2022 |
|
RU2804699C2 |
| METHOD OF INVESTIGATING SECRETORY FUNCTIONS OF NEUTROPHILES | 0 |
|
SU1287006A1 |
| 0 |
|
SU1783435A1 | |
| METHOD OF NATURAL CYTOKINE PREPARING | 1999 |
|
RU2149643C1 |
| PSYCHOTROPIC AGENT | 1989 |
|
RU2008683C1 |
| METHOD OF TESTING METABOLIC ACTIVITY OF BLOOD PHAGOCYTES BY NBT-TEST METHOD | 2012 |
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RU2492484C1 |
| METHOD FOR EVALUATING TOTAL SECRETORY MACROPHAGUE ACTIVITY OF VARIOUS COMPARTMENTS IN CONTINUOUS CULTIVATION | 2011 |
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| METHOD OF NEUTROPHILIC GRANULOCYTE RECOVERY FROM PERIPHERAL BLOOD | 2010 |
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RU2431836C1 |
| METHOD DETERMINATION IMMUNOMODULATED ACTIVITY OF NEUTROPHILES | 0 |
|
SU1716447A1 |
| METHOD OF TREATMENT OF CORNEAL ULCERS | 2006 |
|
RU2324488C1 |
