FIELD: medicine.
SUBSTANCE: method of neutrophilic granulocyte recovery from peripheral blood consists in the fact that 2 ml of whole heparinised venous blood is mixed with sterile physiologic saline in proportions 2/3 and layered on a density bi-gradient of ficoll-verografin solutions with the volume of each gradient making 2 ml. It is followed with centrifugation for 40 minutes at 1500 rpm to observe a neutrophilic granulocyte ring of purity 98-100 % on a gradient border.
EFFECT: use of the method allows recovering a pure fraction of neutrophils from a smaller volume of peripheral blood, reducing a recovery time, and producing a greater percentage of viable cells as compared with existing methods of neutrophil recovery.
2 ex, 3 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PREPARING INTERLEUKIN-8 FROM DONOR BLOOD NEUTROPHILES | 2005 |
|
RU2290948C1 |
| METHOD FOR RECOVERING NEUTROPHILIC GRANULOCYTES FROM BLOOD USING TWO-STEP DENSITY GRADIENT OF IOHEXOL | 2020 |
|
RU2739324C1 |
| METHOD OF OBTAINING TUMOR NECROSIS FACTOR-Α FROM BLOOD NEUTROPHILS OF DONORS | 2022 |
|
RU2804699C2 |
| METHOD FOR DETECTING NEUTROPHILIC TRAPS | 2008 |
|
RU2384844C2 |
| METHOD FOR NORMALIZING ACTIVITY OF NEUTROPHIL SUPEROXIDE DISMUTASE IN NEWBORN CALVES WITH IRON DEFICIENCY | 2017 |
|
RU2675365C1 |
| NORMALIZATION METHOD OF NEUTROPHILES CATALASE ACTIVITY OF NEWBORN CALVES WITH IRON DEFICIENCY | 2016 |
|
RU2618465C1 |
| METHOD FOR SEPARATING MONONUCLEARS FROM THE HUMAN BLOOD | 1993 |
|
RU2061958C1 |
| METHOD FOR ASSESSING EXPOSURE OF FUNCTIONS OF NEUTROPHIL GRANULOCYTES OF PERIPHERAL BLOOD TO LIGHT GENERATED BY LIGHT DIODE LIGHT SOURCES | 2013 |
|
RU2545871C1 |
| METHOD FOR DETECTION OF NEUTROPHIL EXTRACELLULAR TRAPS IN SUPRAVITAL STAINED BLOOD PREPARATION | 2021 |
|
RU2768152C1 |
| METHOD FOR DETERMINING PHAGOCYTIC ACTIVITY OF HUMAN PERIPHERAL BLOOD NEUTROPHILS FROM BIOLUMINESCENCE EXTINCTION DEGREE DATA | 2005 |
|
RU2292553C1 |
