METHOD OF OBTAINING TUMOR NECROSIS FACTOR-Α FROM BLOOD NEUTROPHILS OF DONORS Russian patent published in 2023 - IPC A61K35/15 A61K38/19 C07K14/525 

FIELD: immunology.

SUBSTANCE: invention relates to a method of producing tumor necrosis factor-α from donor blood neutrophils. A method of obtaining tumor necrosis factor-α from blood neutrophils of donors, including the isolation of neutrophils from leukocyte suspension of peripheral blood of donors on a double density gradient of sterile solutions of ficoll-verografin, dilution of neutrophils to a concentration of 5×10⁶ cells/ml, activation of neutrophils by adhesion on polystyrene plastic for 1 hour at a temperature of 37°C with the subsequent separation of the supernatant containing the target product characterized by the following: medium 199 is used to isolate donor blood neutrophils.

EFFECT: above invention is a new simple and affordable way to provide a high level of secretion of TNF-α donor blood neutrophils.

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