PEROXIDE DETERMINATION IN BIOLOGICAL FLUIDS Russian patent published in 2007 - IPC G01N33/84 

FIELD: medicine, biochemistry.

SUBSTANCE: invention relates to method for determination of peroxide group content in lipids containing in biological fluids. Claimed method includes sampling of tested fluid, extraction of organic components by sample treatment with 3-fold amount of chloroform as solvent. After extraction solvent is vaporized in inert gas flow , further total amount of lipids is determined by gravimetric analysis. Then sample is treated with glacial acetic acid up to total protein deposition in flake form. Then reagent mixture comprising KJ and CH₃COOH in ratio of 1.0-1.5 l CH₃COOH per 100 g KJ is introduced into tested sample to provide redox-interaction with globulated protein molecules up to total iodine reducing. Sample is placed in dark room for 1.5-2 h and further released iodine amount is determined by titrimetric method. And on the base of obtained data peroxide group content in lipids is determined.

EFFECT: simplified and accelerated method.

9 ex, 3 tbl