FIELD: medicine, clinical microbiology.
SUBSTANCE: invention proposes a nutrient medium for isolating mycobacteria that contains sodium dihydrogen phosphate, sodium hydrogen phosphate, magnesium sulfate, sodium citrate, iron ammonium citrate, amino acid, agar-agar, glycerol, horse inactivated serum, dye malachite green and distilled water. Addition of α-aminoacetic acid and sodium pyruvate promotes to accelerating growth of mycobacteria with their homogenous distribution in nutrient medium. Horse serum inactivated at 56°C for 40 min decreases its inhibitory effect on mycobacteria. Bactericidal dye malachite green possesses the optimal effect on concomitant microflora but it doesn't delay of mycobacteria growth. Invention provides enhancing growth properties of nutrient medium, accelerates growth of mycobacteria that promotes to they detection for maximally short time. Invention can be used in early diagnosis of tuberculosis.
EFFECT: improved and valuable properties of nutrient medium.
1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PREPARING CULTURE MEDIUM FOR GROWING MYCOBACTERIA | 2008 |
|
RU2403281C2 |
| CULTURE MEDIUM FOR CULTURING L-FORMS OF TUBERCULOSIS MICROBACTERIA | 0 |
|
SU908794A1 |
| NUTRIENT MEDIUM FOR CULTURING MYCOBACTERIUM AND NOCARDIOFORMOUS ACTINOMYCES | 2006 |
|
RU2322495C2 |
| METHOD OF SPECIFIC DIFFERENTIATION OF TUBERCULOSIS MYCOBACTERIA | 2009 |
|
RU2428484C2 |
| DENSE NUTRIENT MEDIUM FOR CULTIVATION OF MYCOBACTERIA, ISOLATED FROM LEPROMAS OF LEPROSY PATIENTS | 2009 |
|
RU2413764C1 |
| NUTRIENT MEDIUM FOR ISOLATION AND CULTURING MYCOBACTERIA FROM BIOLOGICAL MATERIAL | 2006 |
|
RU2315812C1 |
| NUTRIENT MEDIUM FOR DETECTION OF L-FORMS OF MYCOBACTERIA | 2011 |
|
RU2479630C1 |
| METHOD OF DETERMINATION OF SPECIFIC ACCESSORY OF MICOBACTERIA | 2006 |
|
RU2340675C2 |
| PYRAZINEAMIDE-CONTAINING NUTRIENT MEDIUM FOR DETERMINATION OF MEDICINAL RESISTANCE OF TUBERCULOSIS PATHOGEN AND THEIR IDENTIFICATION | 1993 |
|
RU2099417C1 |
| METHOD OF SPECIES INDENTIFICATION OF MYCOBACTERIUM L-FORMS | 0 |
|
SU1687605A1 |
