METHOD OF SPECIFIC DIFFERENTIATION OF TUBERCULOSIS MYCOBACTERIA Russian patent published in 2011 - IPC C12Q1/04 C12N1/20 

FIELD: medicine.

SUBSTANCE: nutrient medium containing twice-substituted potassium phosphate 0.5 g, magnesium sulphate 0.5 g, L-asparagin 4.0 g, chemically pure glycerine 40.0 g, malachite green 20.0 ml, citric acid 2.0 g, citric ammonium iron 0.05 g, agar 10.0 g, hard-boiled yolk 250.0 g and distilled water to 1000 ml is inoculated by a preprocessed diagnostic material. Atypical mycobacteria are differentiated from tuberculosis mycobacteria by observing growth after 1-2 weeks of cultivation, while avian and bovine tuberculosis mycobacteria are differentiated from human mycobacteria after 3 weeks of cultivation. If observing no growth within 10 weeks of cultivation or poor growth, the presence of human tuberculosis mycobacteria are detected and differentiated from bovine tuberculosis mycobacteria by required supplementary cultivation on sodium salicylate medium and TSN medium and gas-liquid chromatography.

EFFECT: simplified method with higher efficiency of differentiation.

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