FIELD: chemistry of hormones.
SUBSTANCE: invention relates to a method for preparing insulin or insulin derivative from insulin precursor or insulin derivative. Precursor is subjected for formation of folded structure in the presence of cysteine or cysteine hydrochloride and a chaotropic accessory substance. Then method involves enzymatic cleavage of precursor with trypsin or trypsin-like enzyme and, if necessary, with carboxypeptidase B additionally followed by purification in adsorbing resin. Method provides preparing insulin or its derivative with correctly joined cystine bridges.
EFFECT: improved preparing method.
18 cl, 4 ex
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