FIELD: medicine, biology, agriculture.
SUBSTANCE: the present innovation deals with fulfilling sorption immunoenzymatic assay to detect small quantities of biomolecules, such as an antigen, an antibody, etc. The innovation, in particular, refers to heating-mediated immobilization of antigen or antibody onto activated surface followed by carrying out further ELISA stages at controlled temperature. The innovation shortens total time required for implementing ELISA by approximately up to 3 h. It is quick, economic, reproducible and simple technique.
EFFECT: higher efficiency.
24 cl, 14 ex, 14 tbl
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