FIELD: biology, medicine.
SUBSTANCE: claimed method includes washing of porcine placenta tissues in phosphate buffer of Hanks solution and grinding thereof to produce pieces of 2-3 cm3. Pieces is poured with 150 ml of 0.25 % tripsin solution, treated in magnetic agitator for 3 h at 20°C. Further mass of regional stem cells is grown in single layer form on medium 199 followed by extraction.
EFFECT: extract containing all regulatory peptides inducing regenerative processes in tissues.
1 ex, 1 tbl
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