FIELD: medicine.
SUBSTANCE: embryonic tissue is flushed with enzyme solution at 25-37°C temperature. For enzyme neutralisation is used medium, made of Eagle medium with lactalbumine hydrolysate or bovine serum.
EFFECT: due to reducing of trypsin proteolitic activity and higher development temperature in stable cell cultures manufacture, yield of viable diploid cells is increased in 1,5-2 times compared to closest analogue of this invention.
7 cl, 4 tbl
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