METHOD FOR HUMAN SALIVARY GLAND CELLS CULTIVATION Russian patent published in 2017 - IPC C12N5/00 

FIELD: biotechnology.

SUBSTANCE: invention is a method for human salivary glands epithelial progenitor cells culturing, comprising: (a) human salivary glands epithelial progenitor cells obtaining from the recipient organism; (b) transfer of cells to the PCT Epidermal Keratinocyte Medium and cultivation in the medium in culture flasks providing cell adhesion at 37°C in the presence of 5% CO₂ with medium replacement every 2-4 days until the cells reach the monolayer; (c) cells passage with 1:3-1:5 dilution, including cells removal from the culture flask surface with tryptic solution in EDTA and transfer to new culture flasks; (d) further cells culturing as described in (b), replacing the medium every 2 to 4 days during cultivation and providing passages upon reaching the monolayer, as described in item (c) with dilution of not more than 1:2-1:3, where the first medium replacement after each passage is performed no later than in 8-24 hours.

EFFECT: increased number of passages of human salivary gland epithelial progenitor cells, maintenance of their undifferentiated state and high proliferative potential in the process of cultivation.

5 cl, 38 dwg, 6 tbl, 2 ex