FIELD: biotechnology, organic chemistry, biochemistry.
SUBSTANCE: invention represents a novel method of preparing optically active 4-(indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid (IHOG) used in preparing monatine, and a method for synthesis of optically active monatine. Also, invention relates to a novel aldolase used in these methods. 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid of high optical purity representing effective intermediate compound for synthesis of optically active monatine can be synthesized from indolpyruvic acid and pyruvic acid (or oxalacetic acid). Invention provides preparing 4-(indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid and monatine with high degree of effectiveness.
EFFECT: improved preparing method.
18 cl, 12 dwg, 12 tbl, 25 ex
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