FIELD: biotechnologies.
SUBSTANCE: invention represents the method for obtaining 2S,4R-monatin or its salt providing the contact of 4R-IHOG with aminotransferase of L-amino acid in presence of L-amino acid and method for 2S,4R-monatin or its salt obtaining with provision of 2S,4R-monatin isomerisation. The present invention also reveals aminotransferases of L-amino acid for performing the methods of monatin obtaining and method for obtaining such L-aminotransferases providing the cultivation of bacterial cell, into which there input is an expression vector containing polynucleotide that codes the presented aminotransferase.
EFFECT: invention allows obtaining 2S,4R-monatin and 2R,4R-monatin with improved output due to the use of revealed aminotransferases of L-amino acid.
27 cl, 6 dwg, 23 tbl, 48 ex
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