FIELD: virology, vaccines.
SUBSTANCE: method involves culturing virus in cells, their lysis, clearing virus-containing liquid, its concentrating and additional purification followed by repeated clearing viral fraction and sorption of viral antigen. Cells lysis is carried out with a nonionic detergent Tween-80 solution, and streptomycin sulfate is used at stage of additional purification. Method provides enhancing the yield of viral antigen. Invention can be used in medicine.
EFFECT: improved preparing method of vaccine.
5 cl, 10 tbl, 3 ex
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