DRY DIAGNOSTIC ANTHRAX ALLERGEN Russian patent published in 2008 - IPC C12P21/02 A61K38/00 G01N33/569 C12R1/07 

FIELD: microbiology, veterinary science, biotechnology.

SUBSTANCE: invention relates to diagnostic immunobiological preparations comprising biologically active components of cells Bacillus anthracis. Method involves submerged culturing the strain B. anthracis, the strain CTI-1 in apparatus BIOP-1,0 in liquid nutrient medium based on hydrochloric hydrolyzate of fish flour for 8-12 h followed by washing out, centrifugation and devitalization of cells. Then alkali-soluble components are extracted from prepared bacterial mass. Borate buffer is added to the substance and dry allergen is prepared in sublimating device LZ-45. Drying temperature regimen is regulated by heat supply to material from -32^°C to +28^°C. The total duration of lyophilization process is 20-24 h. One dose of the prepared dry allergen^{comprises 20 mcg of protein. Biological activity of dry anthrax allergen exceeds the activity of commercial analog by two-fold, it is stable in prolonged storage and effective in estimation of anti-anthrax immunity expression and in diagnosis of anthrax infection.}

EFFECT: enhanced and valuable properties of allergen.

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