METHOD FOR PRODUCING DIAGNOSTIC ANTHRAX ALLERGEN Russian patent published in 2011 - IPC C12P1/04 C12N1/20 A61K39/07 C12R1/07 

FIELD: medicine.

SUBSTANCE: method provides recovery of a diagnostic anthrax allergen directly from a concentrated suspension of vegetative cells of a vaccine strain Bac. anthracis STI-1. The strain is grown on a nutrient medium based on a muriatic hydrolyzate of a fish flour by deep cultivation. The produced bacterial mass is washed in a separator to produce the concentrated suspension of vegetative cells. An allergenic protein fraction is recovered by alkaline hydrolysis, and the prepared hydrolyzate is fractioned. A protein allergen fraction is recovered from a supernatant of the recovered fraction by fractioning with an acetic acid solution; further the precipitation containing an end product is dissolved, dialyzed to produce a purified anthrax protein allergen. The preparation is presented in liquid and lyophilised forms.

EFFECT: method allows higher safety of producing the preparation and reduced length of a technological process, with preserving long-storage activity, effectiveness both in evaluating the anti-anthrax immunity stress, and diagnosing the infection.

4 cl, 4 tbl