EXPRESS-DIAGNOSIS METHOD FOR DETECTING HYPEROXYPROLINURIA CASES Russian patent published in 2008 - IPC G01N33/52 

FIELD: medicine.

SUBSTANCE: method involves taking daily urine sample and its analysis for Oxyprolin available in it. To do it, filtering paper is used for determining Oxyprolin content, one half of the paper being wetted with 7% cholramine B solution and the other one with solution containing 10 g of citric acid, 5 g of recrystallization-refined n-dimethylamine benzaldehyde and 10 ml HCl in 25 ml of water. The paper worked in this way is dried and its end wetted with chloramine B is introduced into daily urine sample. It is expected, when the paper becomes wet to the level wetted with n-dimethylamine benzaldehyde solution. Then the paper is withdrawn and dried in hot air jet. Brown spot arising, hyperoxyprolinuria is considered to be diagnosed, otherwise the diagnosis is considered not to be proved.

EFFECT: simplified test method; no prolonged sample pretreatment required.