FIELD: microbiology. SUBSTANCE: method involves preparing bilayer agar nutrient medium based on Hottinger broth with addition of 2-2.5 g/l yeast extract and 0.30-0.33 g/l calcium chloride. Agar concentration in lower layer is 1.0-1.5 wt.-% and in upper layer is 0.6-0.8 wt.-%. Culture to be analyzed is sown on upper layer and incubated in the presence of human erythrocytes at amount 6-8% of medium volume for 22-24 h. Results are recorded by the presence of erythrocyte hemolysis zones. Invention is used for characterization of strain by their biological activity. EFFECT: enhanced sensitivity, accelerated analysis and colony selection in a single strain. 3 dwg, 3 ex
