FIELD: analytical chemistry, in particular HPLC method for detection of water soluble vitamins in feeds, polyvitamin blends, body fluids (blood serum) and other biological samples.
SUBSTANCE: claimed method includes extraction of tested vitamin from sample of analyzed product, filtration, extract centrifugation and chromatography by HPLC method by using of precolumn, degasser, and thermostat for column, wherein as eluent mixture of methanol and acetate buffer solution with predetermined pH value is used. Methanol/acetate buffer ratio is selected on the base of column type, and wave length is 275 nm for group B vitamins, and 295 nm for vitamin C.
EFFECT: method of increased concentration range and improved accuracy.
8 cl, 1 tbl, 12 dwg, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF DETECTING WATER-SOLUBLE VITAMINS IN PREMIXES | 2009 |
|
RU2409408C2 |
| METHOD OF DETERMINATION OF MEDICINAL FORM COMPOSITION | 1997 |
|
RU2134883C1 |
| METHOD OF VITAMIN B DETERMINATION IN BIOLOGICAL MATERIAL | 1994 |
|
RU2082170C1 |
| METHOD OF QUANTITATIVE DETERMINATION OF SUBSTITUTED CEPHALOSPORINES | 0 |
|
SU1048401A1 |
| METHOD OF MEASURING WEIGHT FRACTION OF L-CARNITINE AND D-CARNITINE IN FODDERS, FORMULATED FEED, FEED ADDITIVES AND MEDICINAL AGENTS FOR ANIMALS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENT DETECTION | 2020 |
|
RU2740535C1 |
| METHOD OF DETERMINING COMPLEX NITROPHENOL PREPARATION "NITRAFEN" IN BIOLOGICAL MATERIAL | 1999 |
|
RU2153169C1 |
| METHOD FOR DETECTION OF OXYBENZENE AND MONOMETHYL DERIVATIVES THEREOF IN BIOLOGICAL SAMPLE | 2004 |
|
RU2269137C1 |
| METHOD OF DETERMINING PROCAINE IN BLOOD PLASMA | 2013 |
|
RU2537179C1 |
| METHOD OF IDENTIFYING AND DETERMINING WEIGHT CONCENTRATION OF ACETALDEHYDE IN ALCOHOL-CONTAINING SOLUTIONS | 1999 |
|
RU2162599C1 |
| METHOD OF DETERMINING 2,4-DICHLOROPHENOXY ACETIC ACID IN BIOLOGICAL MATERIAL | 2011 |
|
RU2453848C1 |
