FIELD: biotechnology, biochemistry, analytical chemistry. SUBSTANCE: examined material containing vitamin B5 is subjected for acid hydrolysis in 1-2 N H2SO4. Obtained hydrolyzate is deproteinized and subjected for chromatography on sorbent Silasorb SPH C18. Vitamin is eluted with a mixture acetic acid : 25% trimethylamine aqueous solution : water at volume ratio = 3: 2:95. EFFECT: improved method of assay. 3 dwg, 3 tbl
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Authors
Dates
1997-06-20—Published
1994-12-30—Filed