FIELD: biotechnology, medicine industry, pharmaceutical industry, cosmetic.
SUBSTANCE: claimed BAS is obtained by purification of milk raw materials by centrifugation, protein sorption on ion exchanger, chromatography separation thereof, eluation, determination of fraction with optical density more than 0.1 units at 280 nm, dialysis, stabilization, and pre-packing. As raw materials milk-originated native cation protein substances are used. Chromatography separation is carried out CM-cellulose, and dialysis is carried out against distilled water or phosphate buffer. Stabilization and microbial purification are carried out by microfiltration trough semipermeable membrane with selectivity of 0.22-0.45 mum. Obtained BAS is pre-packed, frozen to -20-25°C and freeze dried at temperature of -20±4°C. BAS contains fraction of milk-originated native cation protein fraction enriched with lisosime, angiogenine, pancreatic RNAase, lactoferrine and lactoperoxidase.
EFFECT: BAS with anti-microbial, antioxidant, immunomodulatory, anti-stress properties.
2 tbl, 4 ex
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Authors
Dates
2008-03-10—Published
2006-07-07—Filed