METHOD FOR PRODUCTION OF "L-PFI" DIETARY SUPPLEMENT FROM SECONDARY MILK STOCK AND "L-PFI" DIETARY SUPPLEMENT PRODUCED BY THIS METHOD Russian patent published in 2010 - IPC A23L1/30 A23C9/142 A23C9/146 A23C23/00 A23J3/08 

FIELD: food industry.

SUBSTANCE: invention relates to diary, biotechnological, medical, pharmaceutical and cosmetological industries, namely to methods of production of biologically active substances which may be used as dietary supplements. The method includes milk stock purification, separation, caseation, two-stage ultrafiltration, proteins chromatographic separation, dialysis, microfiltration with a 0.2-0.3 mcm pore selectivity, separated monofractions mixture in a ratio which is similar to their content in fresh cow's milk, lyophilisation. Separation of the immunoglobulin fraction from the whey (at the first stage of ultrafiltration) is performed through 100 kilodalton membranes. The permeate is subjected to a second ultrafiltration through 30 kilodaltons membranes. The produced protein concentrate is subjected to ion-exchange chromatography illuated with a 0.35 M - 1 M NaCl gradient with lactoferrin and lactoperoxidase fractions production. Mixture of the produced fractions (immunoglobulin, lactoferrin and lactoperoxidase) is performed in the ratio 91:5:4.

EFFECT: invention allows to produce a dietary supplement containing, in native form, immunoglobulins, lactoperoxidase and lactoferrin; the dietary supplement has immunomodulatory, antioxidative, antistress, antibacterial and general tonic actions.

2 cl, 1 tbl, 1 ex