FIELD: medicine.
SUBSTANCE: method involves carrying out conventional mice hyperimmunization of BAlb/c for producing polarized lymphocytes with three-fold subcutaneous ovalbumin introduction at a dose of 100 mcg/mouse and aluminum hydroxide adjuvant introduction at a dose of 5 mg/mouse making 14 days long pause between injections. Spleens are taken and lymphocytes are separated 7 days later after the last injection.
EFFECT: simplified method for obtaining polarized TH-2 lymphocytes in the amount for modeling immune response of TH-2 type.
4 tbl
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APPLICATION OF MYCOBACTERIUM VACCAE FOR FEEDBACK INHIBITION REGULATION OF TH2-ACTIVITY OF IMMUNE SYSTEM | 1997 |
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Authors
Dates
2008-03-10—Published
2006-06-29—Filed