MICROORGANISMS AS CARRIERS OF NUCLEOTIDE SEQUENCES ENCODING CELLULAR ANTIGENS FOR TREATMENT OF TUMORS Russian patent published in 2008 - IPC C12N1/21 C12N15/63 A61K35/74 A61P43/00 

FIELD: microbiology, genetic engineering, medicine, oncology.

SUBSTANCE: invention relates to genetically modified microorganism comprising the following components in its genomes that are expressed: (I) nucleotide sequence encoding at least one the tumor cell antigen epitope, and/or nucleotide sequence of at least one antigen epitope showing specificity to tissue cell generating tumor; (II) nucleotide sequence encoding protein that stimulates cell of the immune system; (IIIA) nucleotide sequence of the transport system providing expression of product of components (I) and (II) expression on external surface of microorganism, and/or secretion of product of component (I) and component (II) expression, and/or (IIIB) nucleotide sequence of protein for lysis of microorganism in mammal cells cytosol and for intracellular releasing plasmas containing in microorganisms to be lysed, and (IV) activating sequence activated in microorganism showing specificity to tissue cell but not specific to cell for expression of one or some components from (I) to (IIIB), and wherein each of components from (I) to (IV) can be represented once or many times. Proposed microorganism is useful for using as a drug as component of anti-tumor vaccine. Also, invention describes plasmid or the expression vector for preparing the genetically modified microorganism and a method for its preparing. Vaccine based on genetically modified microorganism represents the improved and overcome immune tolerance with respect to tumors in case for its using for prophylaxis and therapy of tumors.

EFFECT: valuable properties of microorganisms.

21 cl, 1 dwg, 2 ex