FIELD: medicine, genetic therapy. SUBSTANCE: invention relates to method for preparing genetically modified cells. Method involves isolation of cells from blood or body liquids, culturing prepared cells in medium for cells culturing containing gangliosides, phospholipids, glycolipids and/or endothelial cells growth factors, by optional, immortalization of prepared cells by their transformation with oncogene taken among group consisting of mutated genes cdk-4, cdk-6 and cdk-2, activation of protooncogene or inactivation of suppressor gene, transfection of prepared cells using construction of nucleic acid for genetic therapy comprising gene-effector that can be activated with using useful promoter systems specifically to cell, specifically to mitosis, specifically to virus and/or by hypoxia. The advantage of method is the development of endothelial cells as cellular carriers of transgenes in genetic therapy. EFFECT: valuable medicinal properties of cells. 18 cl, 1 dwg, 2 tbl
