METHOD FOR ACCELERATED ASSAY OF SENSITIVITY OF BURCKHOLDERIAE TO CHEMOPREPARATIONS Russian patent published in 2008 - IPC C12Q1/04 

FIELD: microbiology.

SUBSTANCE: invention relates to an accelerated method for assay of sensitivity of burkcholderiae to chemopreparations. Accelerated assay method is based on using a solid nutrient medium with an indicator. Medium contains tryptone and glucose with indicator bromothymol blue, pH 6.0-7.6 (acid - yellow color, alkaline - blue color), the parent pH of medium is 6.8, and method involves preliminary preparing burkcholderiae to be analyzed before their inoculation. For carrying out the assay a medium is prepared of the following composition, g/l: tryptone, 2.0; NaCl, 5.0; K₂HPO₄, 0.3; bromothymol blue, 0.08; agar, 15.0, and distilled water, up to 1 l, pH 6.8. Sterile glucose solution (10 g/l) is added to medium before pouring plates at temperature 45^°C and poured by a layer of thickness 4.0 ± 0.5 mm before 24 h for carrying out the assay. Analyzed culture of microorganisms is diluted to the concentration 10⁸ cells/ml in 0.85% NaCl heated to 37^°C and maintained this temperature up to inoculation moment. Then suspension of analyzed strain of burkcholderiae is applied on heated plates with medium in the amount 10⁸ cells in volume of 0.2 ml of water. Then disks impregnated with antibiotics are places on agar surface. After 3-5 h of incubation (depending on the pathogen species) result is recorded: microorganisms retention zone growth around disks remain of green-like color but in zones of cultures growth without antibiotics medium turn yellow based on acidification of medium as result of glucose destruction by multiplying microorganisms. Method provides accelerating assay of sensitivity of burkcholderiae to chemopreparations, significant decrease for recording results on solid nutrient medium with indicator as compared with using the standard medium (3-5 h and 18-24 h, respectively).

EFFECT: improved assay method.

2 cl, 2 tbl, 2 dwg, 2 ex