METHOD OF SELECTION OF HIGHLY ACTIVE ANTIBACTERIAL MEDICATION FOR TREATMENT OF DISEASES CAUSED BY PATHOGENIC BURKHOLDERIA Russian patent published in 2010 - IPC C12Q1/02 C12Q1/04 C12R1/01 

FIELD: medicine.

SUBSTANCE: tested culture of B. pseudomallei or B. Mallei in dose 10⁴ mc is inoculated on Muller Hinton medium, disc, usually applied for treatment of acute forms of melioidosis (ceftazidime, meropenem, ciprofloxacin, doxycycline, rifampicillin, chloramphenicol, co-trimoxazole) are added and cultivated at 37°C for 24 hours. Simultaneously the same concentrations of tested cultures are inoculates on the same medium with addition of 10% of blood of laboratory animals - golden hamsters, discs are applied and cultivated at 37°C in atmosphere, containing 5% CO₂, for 24 hours, after which results are counted. As index of antibacterial medication activity serves preservation of activity in conditions close to in vivo, at the level of bacteria growth indexes in standard conditions, zone of growth retardation essentially exceeding index of bacteria sensitivity to said antibacterial medication. Simultaneously determined is comparative efficiency of medications in experiments on treatment of experimental animals.

EFFECT: invention allows to determine which antibacterial medication is efficient for treatment of diseases caused by pathogenic burkholderias more accurately.

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