FIELD: biology, cellular engineering.
SUBSTANCE: method involves addition of lanthanum chloride to barley cell protoplasts to its final concentration 5-10 mM, and after aggregation protoplasts are incubated at 42°C for 30 min. Amount of fused protoplasts is carried out using light microscope. Protoplast fusion frequency is estimated to be 30-50%. Invention can be used for preparing large amount of barley cell fused protoplasts.
EFFECT: improved method for fusion of protoplasts.
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