Show metadata Hide metadata

(19)

(11)

2 321 424

(13)

(51)

IPC

A61K48/00(2006-01-01)

C12N15/70(2006-01-01)

A61P35/00(2006-01-01)

(21) (22)

Application

2006138034/15, 2006-10-30

(24)

Start date

2006-10-30

(22)

Actual filing date

2006-10-30

(45)

Published

2008-04-10

(72)

Inventor

Jarotskij Sergej ViktorovichChuvpilo Sergej Al'BertovichSkrypin Vasilij IvanovichMogutov Mikhail AleksandrovichJakovenko Andrej Romanovich

(73)

Holder

Obshchestvo S Ogranichennoj Otvetstvennost'Ju

PREPARATION, RECOMBINANT PLASMID DNA pSX70 ENCODING SYNTHESIS OF HUMAN RECOMBINANT GRANULOCYTE-COLONY-STIMULATING FACTOR (G-CSF), Escherichia coli SX70 STRAIN AS PRODUCER OF HUMAN RECOMBINANT G-CSF AND METHOD FOR INDUSTRIAL PREPARING G-CSF Russian patent published in 2008 - IPC A61K48/00 C12N15/70 A61P35/00

Abstract RU 2321424 C1

FIELD: biopharmacology, biotechnology, microbiology.

SUBSTANCE: invention relates to methods for industrial preparing the human recombinant granulocyte-colony-stimulating factor (G-CSF) of medicinal designation, and to recombinant Escherichia coli (E. coli) strains and plasmids used for its preparing. Group of inventions involves creature of recombinant plasmid DNA pSX70, constructing recombinant industrial Escherichia coli pSX70 strain-producer deposited in All-Russian collection of Industrial Microorganisms (VKPM), FGUP Gos. NII Genetics at collection number VKPM B-8842. Invention provides preparing G-CSF with the high yield by relatively simple and safety technology that can be used as a base for preparing composition in form of aqueous solution for injection.

EFFECT: improved preparing method, valuable biological properties of strain.

9 cl, 1 dwg, 3 ex

Similar patents RU2321424C1

Title	Year	Author	Number
METHOD FOR PREPARING RECOMBINANT HUMAN ALPHA-2B-INTERFERON, RECOMBINANT PLASMID AND STRAIN-PRODUCER FOR ITS REALIZATION	2003	Cherepanov P.A. Mikhajlova T.G. Cherepanov P.P.	RU2242516C1
RECOMBINANT PLASMID pFM-GCSF5 THAT PROVIDES EXPRESSION OF SYNTHETIC HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR, ESCHERICHIA COLI FM-GCSF BACTERIA STRAIN - PRODUCER OF RECOMBINANT HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR AND METHOD FOR PRODUCTION THEREOF	2018	Markov Ilya Aleksandrovich Markova Elena Alekseevna Gaponyuk Polina Petrovna Markova Inna Nikolaevna	RU2680886C1
SOLUTION FOR INJECTION, RECOMBINANT PLASMID DNA pSX50 ENCODING SYNTHESIS OF HUMAN RECOMBINANT ALPHA-2b INTERFERON, STRAIN Escherichia coli SX50 AS INDUSTRIAL STRAIN-PRODUCER OF HUMAN RECOMBINANT ALPHA-2b INTERFERON AND METHOD FOR INDUSTRIAL PREPARING INTERFERON ALPHA-2b	2006	Mogutov Mikhail Aleksandrovich Jarotskij Sergej Viktorovich Skrypin Vasilij Ivanovich Jakovenko Andrej Romanovich Chezhgalova Nelli Anatol'Evna Selishchev Stepan Vladimirovich	RU2319502C1
RECOMBINANT PLASMID DNA PSS5 ENCODING SYNTHESIS OF RECOMBINANT HUMAN ALPHA-2B INTERFERON, STRAIN OF ESCHERICHIA COLI SS5 AS PRODUCER OF RECOMBINANT HUMAN ALPHA-2B INTERFERON AND METHOD OF ALPHA-2B INTERFERON PREPARING	1999	Cherepanov P.A. Mikhajlova T.G. Cherepanov P.P. Martinenko Dmitrij Leonidovich Shevchuk Aleksandr Anatol'Evich Fedjukin V.S. Nikolaev T.M. Tolkachev B.B. Sventitskij E.N. Urakov N.N. Kalinin Ju.T. Denisov L.A. Tjagotin Ju.V. Martjushin S.V. Ishchenko A.M. Trofimov A.V. Poljakova E.A. Batarin V.I. Shalaeva O.N. Lisitskaja V.I.	RU2165455C1
RECOMBINANT PLASMID DNA PES3-7 ENCODING PEPTIDE WITH SEQUENCE OF HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR (G-CSF) AND STRAIN ESCHERICHIA COLI BL21(DE3)/PES3-7 AS PRODUCER OF HUMAN RECOMBINANT G-CSF	2003	Gabibov A.G. Ponomarenko N.A. Vorob'Ev I.I. Demin A.V. Mart'Janov V.A. Shuster A.M. Bairamashvili D.I. Miroshnikov A.I.	RU2260049C2
RECOMBINANT DNA, CODING HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR (G-CSF) AND RECOMBINANT PLASMID pAS017, PROVIDING G-CSF SYNTHESIS IN CELLS OF Escherichia coli	2011	Shul'Ga Aleksej Anatol'Evich	RU2529363C2
RECOMBINANT PLASMID DNA PTTG KM2 ENCODING SYNTHESIS OF RECOMBINANT HUMAN IMMUNE INTERFERON, STRAIN ESCHERICHIA COLI T3G - A PRODUCER OF RECOMBINANT HUMAN IMMUNE INTERFERON AND A METHOD OF PREPARING RECOMBINANT HUMAN IMMUNE INTERFERON	1996	Cherepanov P.A. Pavlov V.M. Fedjukin V.S. Shmatchenko N.A. Astashkina G.F. Vorotnikova I.I. Gavrikov V.G. Bajdus' A.N. Mikhajlova T.G. Denisov L.A. Urakov N.N. Stepanov A.V.	RU2097428C1
RECOMBINANT PLASMID PFM-IFN-17 PROVIDING EXPRESSION OF INTERFERON ALPHA-2B OF HUMAN, RECOMBINANT PLASMID PFM-AP, PROVIDING EXPRESSION OF ENZYME METHIONINE-AMINOPEPTIDASE E COLI, BIPLASMID STRAIN ESCHERICHIA COLI FM-IFN-AP (PFM-IFN-17, PFM-AP) - PRODUCER (MET-) OF RECOMBINANT INTERFERON ALPHA -2B OF HUMAN	2016	Markov Ilya Aleksandrovich Markova Elena Alekseevna Gaponyuk Polina Petrovna Markova Inna Nikolaevna	RU2610173C1
METHOD FOR PRODUCING OF HUMAN METHIONINE-FREE INTERFERON-ALPHA2b	2009	Shirokov Dmitrij Alekseevich Rjabichenko Viktor Vasil'Evich Akishina Raisa Illarionovna Glazunov Aleksandr Viktorovich Chestukhina Galina Georgievna Vejko Vladimir Petrovich	RU2432401C2
RECOMBINANT DNA PLASMID PGSDE1 ENCODING TICK-BORNE ENCEPHALITIS VIRUS PROTEIN E AND STRAIN OF ESCHERICHIA COLI - PRODUCER OF TICK-BORNE ENCEPHALITIS VIRUS PROTEIN E	1997	Netesova N.A. Belavin P.A. Malygin Eh.G. Rukavishnikov M.Ju.	RU2136754C1

RU 2 321 424 C1

Authors

Jarotskij Sergej Viktorovich

Chuvpilo Sergej Al'Bertovich

Skrypin Vasilij Ivanovich

Mogutov Mikhail Aleksandrovich

Jakovenko Andrej Romanovich

Dates

2008-04-10—Published

2006-10-30—Filed