FIELD: biotechnology, genetic engineering. SUBSTANCE: invention involves the development of recombinant plasmid DNA pGSDE1 encoding the envelope protein E of tick-borne encephalitis virus and the strain of Escherichia coli as a producer of protein E. Recombinant plasmid DNA pGSDE1 is constructed on the basis of HindIII-ScaI-fragment of plasmid pEZZT 318 (size is 1165 base pairs), ScaI-SacI-fragment of plasmid pGEM1 (size is 1640 base pairs), SacI-BglII-synthetic fragment (size is 12 base pairs) and BglII-HindIII-fragment obtained by method of polymerase chain reaction (size is 1250 base pairs) that contains starting ATG-codon, encoding site of gene for protein E of tick-borne encephalitis virus and terminating TAA-codon also. The strain Escherichia coli GSDE - a producer of protein E of tick-borne encephalitis virus is obtained by transformation of plasmid DNA pGSDE1 carrying the gene E of tick-borne encephalitis virus. Recombinant protein can be used as an antigen in diagnostic test-systems in microbiological industry and medicine for development of highly effective prophylactic and diagnostic agents for control of tick-borne encephalitis virus. EFFECT: plasmid and the bacterial strain indicated above. 3 cl, 3 ex
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID DNA PC-NS3 PROVIDING INTEGRATION OF COMPLEX OF GENES C, PRM, E, NS1, NS 2A, NS 2B, NS3 OF TICK-BORNE ENCEPHALITIS VIRUS IN GENOME OF BOVINE VACCINE VIRUS AND RECOMBINANT STRAIN OF BOVINE VACCINE VIRUS EXPRESSING COMPLEX OF GENES C, PRM, E, NS1, NS 2A, NS2B, NS3 OF TICK-BORNE ENCEPHALITIS VIRUS IN IMMUNIZED ORGANISM CELLS |
1995 |
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1998 |
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2000 |
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2018 |
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|
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2008 |
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1993 |
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1998 |
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1999 |
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