FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular to the recombinant plasmid pFM-GCSF5, which encodes the expression of human granulocyte colony-stimulating factor (hereinafter referred to as G-CSF), to the recombinant strain of Escherichia coli FM-GCSF – producer of recombinant human G-CSF, as well as to a method for producing recombinant human G-CSF. Recombinant strain of the E. coli FM-GCSF bacterium is obtained by transforming cells of the E. coli BL21 strain with recombinant plasmid DNA pFM-GCSF-5. pFM recombinant plasmid DNA, GCSF-5, measuring 3335 bp, codes for the synthesis of human G-CSF under the control of the lactose and tryptophan promoter and the transcription terminator ggpVT1T2 and contains the kan synthetic gene, which determines resistance to kanamycin, as a genetic marker.
EFFECT: invention makes it possible to produce recombinant human G-CSF in high yield and by simplified technology.
3 cl, 7 dwg
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