RECOMBINANT DNA, CODING HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR (G-CSF) AND RECOMBINANT PLASMID pAS017, PROVIDING G-CSF SYNTHESIS IN CELLS OF Escherichia coli Russian patent published in 2014 - IPC C12N15/27 C12N15/70

Abstract RU 2529363 C2

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to field of biotechnology, namely to recombinant obtaining G-CSF, and can be used for production of G-CSF in cells of E.coli. For effective production of protein in cells of E.coli G-CSF-coding DNA sequence is optimised. On the basis of obtained optimised DNA sequence plasmid pAS017, also including NdeI/BamHI-fragment of DNA of pETM-50 vector and having physical map, presented on the drawing, is constructed.

EFFECT: invention provides effective production of protein in cells of Ecoli.

2 cl, 1 dwg, 1 tbl, 3 ex

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RU 2 529 363 C2

Authors

Shul'Ga Aleksej Anatol'Evich

Dates

2014-09-27—Published

2011-03-02—Filed