FIELD: biotechnology, biochemistry, molecular biology.
SUBSTANCE: invention represents eucaryotic cell-host that expresses the first polynucleotide encoding the first propeptide and FVII or its functional variants and relates to the first expression unit, and expresses the second polynucleotide encoding the second free propeptide. Each of indicated the first and second propeptides comprises amino acid sequence chosen independently from group consisting of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17 and 18. Also, invention relates to a method for preparing vitamin K-dependent proteins, among them and FVII, using abovementioned cell-host, and to a method for preparing this cell-host. Invention provides preparing vitamin K-dependent proteins with the high degree of effectiveness.
EFFECT: improved preparing method of proteins.
38 cl, 3 tbl, 3 dwg, 2 ex
