FIELD: medicine.
SUBSTANCE: vitamin K dependent protein is made by separating a cultivated eukaryotic cell that contains an expressing vector that contains a nucleic acid molecule coding vitamin K dependent protein and associated sequences regulating expression. The associated sequences contain the first promoter and the nucleic acid molecule coding gamma-glutamylcarboxylase, and the second promoter. The first promoter represents a pre-early promoter of human cytomegalovirus (hCMV), and the second promoter is a pre-early promoter SV40. Herewith the expressing relation of vitamin K dependent protein and gamma-glutamylcarboxylase is 10:1 to 250:1.
EFFECT: invention allows for making gamma-carboxylated vitamin K dependent protein in production quantities.
29 cl, 5 dwg, 6 tbl, 7 ex
| Title |
Year |
Author |
Number |
| HOST CELL CONTAINING VECTOR FOR PRODUCING PROTEINS REQUIRING GAMMA-CARBOXYLATION |
2006 |
|
RU2415934C2 |
| METHOD FOR PREPARING VITAMIN K-DEPENDENT PROTEINS |
2001 |
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- Nil'Sen Lars Seehgor
|
RU2321633C2 |
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2012 |
- Grigor'Eva Ol'Ga Vasil'Evna
- Zaval'Nyj Mikhail Aleksandrovich
- Karpov Andrej Pavlovich
- Petrov Andrej Vladimirovich
- Fabrichnyj Igor' Pavlovich
- Shuster Aleksandr Mikhajlovich
|
RU2500816C1 |
| PLASMID FOR EXPRESSION IN CELL OF CHINESE HAMSTER, CELL OF CHINESE HAMSTER - PROTEIN PRODUCER WITH Gla-DOMAIN, AND METHOD FOR OBTAINING PROTEIN WITH Gla-DOMAIN |
2013 |
- Orlova Nadezhda Aleksandrovna
- Kovnir Sergej Vladimirovich
- Vorob'Ev Ivan Ivanovich
|
RU2535871C1 |
| PURIFICATION OF POLYPEPTIDES |
2009 |
|
RU2544860C2 |
| METHOD FOR PURIFYING VITAMIN K DEPENDENT PROTEINS SUCH AS COAGULATION FACTOR IX |
2011 |
- Gillyam Gustav
- Vinge Stefan
|
RU2590726C2 |
| METHOD OF PURIFYING VITAMIN K-DEPENDENT PROTEINS, SUCH AS COAGULATION FACTOR VII |
2011 |
- Gillyam Gustav
- Vinge Stefan
|
RU2731720C2 |
| EXPRESSION PLASMID VECTOR OF MONOCISTRONIC EXPRESSION OF RECOMBINANT PROTEINS IN MAMMALIAN CELLS, CELL LINE OF MAMMALS-PRODUCERS OF RECOMBINANT PROTEIN, METHOD OF OBTAINING RECOMBINANT PROTEIN |
2013 |
- Orlova Nadezhda Aleksandrovna
- Kovnir Sergej Vladimirovich
- Vorob'Ev Ivan Ivanovich
|
RU2552170C2 |
| PLASMID FOR EXPRESSION OF HUMAN RECOMBINANT BLOOD CLOTTING FACTOR IX, CHO CELL PRODUCING HUMAN RECOMBINANT BLOOD CLOTTING FACTOR IX AND METHOD OF PRODUCING SAID FACTOR |
2014 |
- Kovnir Sergej Vladimirovich
- Orlova Nadezhda Aleksandrovna
- Vorobev Ivan Ivanovich
- Khodak Yuliya Vladimirovna
- Dronina Mariya Alekseevna
- Smirnov Ivan Vitalevich
- Ponomarenko Natalya Aleksandrovna
- Skryabin Konstantin Georgievich
- Gabibov Aleksandr Gabibovich
|
RU2585532C2 |
| METHOD FOR PRODUCING IMMORTALISED HUMAN CELL, STABLE TRANSFECTED IMMORTALISED HUMAN CELL, METHOD FOR RECOMBINANT PRODUCTION OF TARGET HUMAN PROTEIN, APPLICATION OF TRANSFECTION VECTOR |
2006 |
- Shreder Karola
- Vegmann Ketlin
- Din Khajjan'
|
RU2453597C2 |
