FIELD: medicine.
SUBSTANCE: invention presents an eukaryotic host cell for producing a gamma-carboxylated protein, containing an expression vector containing a nucleic acid molecule coding the protein requiring gamma-carboxylation, and associated expression control sequences, and the expression vector containing a nucleic acid molecule coding vitamin K-epoxide reductase and the associated expression control sequences. Besides, the host cell contains the nucleic acid molecule coding γ-glutamyl carboxylase and the associated expression control sequences. The given invention also relates to a method for producing gamma-carboxylated protein and to a method for preparing a pharmaceutical composition effective for blood coagulation induction or hypercoagulability or hypocoagulability stimulation, based on the produced gamma-carboxylated protein.
EFFECT: invention allows high effective production of the gamma-carboxylated protein.
20 cl, 2 dwg, 9 tbl, 5 ex
| Title |
Year |
Author |
Number |
| EUKARYOTIC HOST CELL FOR EXPRESSION VITAMIN K DEPENDENT PROTEIN, EXPRESSION VECTOR IN EUKARYOTIC CELLS, METHOD FOR MAKING GAMMA-CARBOXYLATED VITAMIN K DEPENDENT PROTEIN AND METHOD FOR MAKING PHARMACEUTICAL COMPOSITION FOR COAGULATION INDUCTION OR STIMULATION OF COAGULATION INCREASE OR DECREASE |
2004 |
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- Levgren Ann
- Telin Anders
|
RU2372401C2 |
| METHOD FOR PREPARING VITAMIN K-DEPENDENT PROTEINS |
2001 |
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|
RU2321633C2 |
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2013 |
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|
RU2535871C1 |
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2014 |
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|
RU2585532C2 |
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2012 |
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- Zaval'Nyj Mikhail Aleksandrovich
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- Fabrichnyj Igor' Pavlovich
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|
RU2500816C1 |
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2010 |
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|
RU2563231C2 |
| PURIFICATION OF POLYPEPTIDES |
2009 |
|
RU2544860C2 |
| COMPOSITIONS COMPRISING HETEROGENEOUS POPULATIONS OF RECOMBINANT HUMAN CLOTTING FACTOR Xa PROTEINS |
2014 |
- Jankowski Michael Anthony
- Johnson Keith A.
- Piacenza Wendy Carol
- Rouse Jason C.
- Shamashkin Michael
- Sharpe Penelope Jane
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- Weston Stacey B.
|
RU2648144C2 |
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2011 |
|
RU2570547C2 |
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2006 |
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|
RU2453597C2 |
