METHOD OF QUANTITATIVE DETERMINATION OF PHTHALIC ACID IN BLOOD SERUM Russian patent published in 2008 - IPC G01N33/50 

FIELD: medicine; diagnostic techniques.

SUBSTANCE: proposed method consists in sampling of 2 cm³ serum, which contains phthalic acid; following acidation with aqueous solution of acetic acid till pH 3; the solid-state extraction at Oasis HLB sorbent is performed with the use of extractant - mixture of methyl and isopropyl alcohol in volume ratio 9:1. Then, the resulted extract is analysed by liquid chromatography, where the mixture of acetonitrile and water in volume ratio 15:85 is used following acidation with aqueous solution of orthophosphoric acid till pH 3. The amount of phthalic acid is determined by using the calibrating plot.

EFFECT: precision of phthalic acid test in presence of benzoic acid is increased; test time is reduced.

3 cl, 3 tbl, 2 ex