METHOD FOR DETERMINATION OF MASS CONCENTRATIONS OF PHENOL AND PYROCATECHOL IN BLOOD BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Russian patent published in 2022 - IPC G01N30/02 

FIELD: analytical chemistry.

SUBSTANCE: invention relates to the field of analytical chemistry. The method for determining the mass concentrations of phenol and pyrocatechol in the blood by high-performance liquid chromatography is characterized by taking a blood sample, acidifying it with a 0.1% solution of orthophosphoric acid in a sample/acid solution volume ratio of 20:1, extracting liquid analytes extraction, for which acetonitrile is added to the acidified sample in a sample/cetonitrile volume ratio of 1:1, respectively, the mixture is centrifuged for 10 min at a speed of 5000 rpm, the upper layer of the extract is taken, 0.1 g of a mixture of C18 sorbent and salts of magnesium sulfate in a mass ratio of 1:6, respectively, to purify the extract, mix for 1 min and centrifuge again for 10 min at a speed of 5000 rpm, the purified extract is filtered and injected into the chromatograph 10 mm³ of the filtered extract, the measurement is carried out and the concentration of phenol and pyrocatechol is determined using a calibration curve.

EFFECT: invention provides the possibility of determining phenol and catechol from one blood sample in their joint presence, while ensuring the high sensitivity of the method.

1 cl, 8 tbl