METHOD OF EVALUATING BIOLOGICAL VALUE OF ANTHOCYAN DYE, OBTAINED USING ACID-FREE METHOD Russian patent published in 2008 - IPC C09B61/00 

Abstract RU 2329284 C2

FIELD: chemistry.

SUBSTANCE: invention pertains to the food industry, and particularly to the method of evaluating the biological value of anthocyan dyes. The method involves preparing an alcohol solution of the analysed dye 1-4 mcl of the obtained solution is placed on the starting line of a chromatographic sheet with a layer of silica gel. After the sample has dried, ascending chromatography is carried out in a system of the following solvents: n-amyl alcohol-acetic acid-water, taken in a ratio of 2:1:1. The sheet is dried, treated with a 1% solution of aluminium chloride in ethanol and examined in UV-light. Evaluation of biological value of the dye is done on the presence of flavonoids in it, found on the zones fluorescent in UV-light.

EFFECT: invention allows increasing sensitivity of the method.

6 ex

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RU 2 329 284 C2

Authors

Selemenev Vladimir Fedorovich

Lomova Tat'Jana Sergeevna

Bolotov Vladimir Mikhajlovich

Rudakov Oleg Borisovich

Nazarova Aleksandra Aleksandrovna

Dates

2008-07-20Published

2006-06-15Filed