METHOD FOR QUANTITATIVE DETERMINATION OF RUTIN VIA THIN-LAYER CHROMATOGRAPHY Russian patent published in 2012 - IPC G01N30/92 

Abstract RU 2469316 C1

FIELD: chemistry.

SUBSTANCE: method for quantitative determination of rutin involves dissolving the analysed preparation sample while heating in ethanol, followed by chromatography using Sorbfil silica gel plates, eluent - ethyl acetate-glacial acetic acid-water (7.5:1.5:1.5); developer - 5% alcohol solution of NaOH; after developing the chromatographic zones, the plates are scanned by an EPSON PERFECTION 2480 PHOTO flatbed scanner and the obtained images are processed using Sorbfil Videodensitometer software, and content of rutin in the analysed sample (c, %) is calculated using the formulae: where S is the surface area of the chromatographic zone on the chromatogram, calculated using the Sorbfil Videodensitometre software.

EFFECT: rapidness, affordability, sufficient sensitivity, selectivity.

4 dwg

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RU 2 469 316 C1

Authors

Checheta Ol'Ga Valer'Evna

Safonova Elena Fedorovna

Slivkin Aleksej Ivanovich

Safonova Irina Igorevna

Dates

2012-12-10Published

2011-05-06Filed